THE NANO-MICROSCOPY FOR THE DIAGNOSIS OF CHRONIC URINARY INFECTION

Objective

In each application, the CytoViva Nano-scale Microscope and Hyperspectral Imaging Systems work together as a seamless solution to provide analytical confirmation of nano-scale materials and their interactions with biologicals or other composite materials. Detection and characterization of bacteria and mycetes in the high and low urinary system. The nano-microscopy technology is easily performed for the urinary sample and for blood sample.This method allowed to valuate the low bacterial load (negative urine culture and/or negative hemoculture) and modified bacteria by resistance antibiotic. Is utilized CytoViva's patented illumination technology which integrates onto a standard optical microscope and create a high signal-to-noise, darkfield-based image. This specialized capability enables fast observation of a wide range of nanomaterials and pathogens along with unlabeled or fluorescently labeled cells and tissue.

Methods and results

The CytoViva Hyperspectral Imaging System attaches to the CytoViva Microscope System. It serves to quantify the presence of a wide range of nanomaterials in cells and tissue or in composites. The system captures the VNIR (400-1000nm) spectrum within each pixel of the scanned field of view. Advanced analytical software then provides detailed spectral analysis of the scanned materials.
For the first time, using this technology is possibile to esaminate live sample and capture immediately images and films.
The cytoviva hyperspectral imaging system allow to achieve a multifunctional result: a target therapy by specific drugs or, in case of resistence, by phitotherapic extracts tested on resistant pathogen.
It have been tested 80 female patients affected by hypogastric pain. Blood/urine samples and material fecal are been analyzed.

In the eighty percent of cases it has been detected a low load microorganism (E. coli, mycoplasma, Candida spp) and it has been studied a targeted therapy.
It has been achieved a completely disappearing of microorganisms and a completely resolution of patient’s disease.

Discussion

This initial work allow us to discover the low bacterial load not detected in other ways.
There is a work in progress for the data capture that it allow us to do a run out of chronic urinary infection. Such analysis could increase the specific antibiotic therapy and phitoterapy successfully.
In particular, the “aromatogramma” is an in vitro method of measurement of the essential oils’s (OE) antibacterial activity: it expresses the antimicrobial activity of the active ingredient to a particular pathogenic strain of bacteria or mycetes present in the biological sample.
The technique used is similar to the antibiogram: measuring the inhibition of growth of the pathogen establishes the degree of sensitivity (high, medium, low), under which it will be possible to formulate a targeted therapy with OE most effective.

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